HEXIM2 Polyklonaler Antikörper

HEXIM2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16598-1-AP

Synonyme

Protein HEXIM2, L3, Hexamethylene bis-acetamide-inducible protein 2

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  WB analysis of C6 using 16598-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 16598-1-AP (HEXIM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16598-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16598-1-AP (HEXIM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 16598-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16598-1-AP (HEXIM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 16598-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16598-1-AP (HEXIM2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16598-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16598-1-AP (HEXIM2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 16598-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16598-1-AP (HEXIM2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16598-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16598-1-AP (HEXIM2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human urothelial carcinoma using 16598-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 16598-1-AP (HEXIM2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 16598-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 16598-1-AP (HEXIM2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 16598-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using HEXIM2 antibody (16598-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen, COLO 320-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Urothelkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16598-1-AP bindet in WB, IHC, IF/ICC, ELISA HEXIM2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HEXIM2 fusion protein Ag9754
• Vollständiger Name: hexamthylene bis-acetamide inducible 2
• Berechnetes Molekulargewicht: 286 aa, 32 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC025970
• Gene symbol: HEXIM2
• Gene ID (NCBI): 124790
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.