HEY1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HEY1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(61)

Rezensionen (1)

Kat-Nr. : 19929-1-AP

Synonyme

HESR 1, HERP2, CHF-2, CHF2, CHF 2

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• 

  WB analysis using 19929-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19929-1-AP (HEY1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19929-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19929-1-AP (HEY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, A549-Zellen, NCI-H1299-Zellen, Rattenhodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 53 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 4 publications below

Produktinformation

19929-1-AP bindet in WB, IHC, IF, IP, CoIP, ChIP, ELISA HEY1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HEY1 fusion protein Ag13906
• Vollständiger Name: hairy/enhancer-of-split related with YRPW motif 1
• Berechnetes Molekulargewicht: 304 aa, 33 kDa
• Beobachtetes Molekulargewicht: 32-34 kDa
• GenBank-Zugangsnummer: BC001873
• Gene symbol: HEY1
• Gene ID (NCBI): 23462
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hairy/enhancer of split-related proteins are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. And HEY1 is one of such protein. It is the direct downstream effector of Notch signaling which may be required for cardiovascular development. It preferentially bind to the canonical E box sequence 5'-CACGTG-3' and acts as a transcriptional repressor. HEY1 is a 33kDa protein and forms dimer by self-associating.

Publikationen

Species	Application	Title
human,mouse	WB	Cell Metab; Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer; Authors - Yu Jiang
human	IHC	Protein Cell; Microbiota enterotoxigenic Bacteroides fragilis-secreted BFT-1 promotes breast cancer cell stemness and chemoresistance through its functional receptor NOD1; Authors - Wei Ma
mouse	WB	Theranostics; Nicotinamide mononucleotide enhances fracture healing by promoting skeletal stem cell proliferation; Authors - Yitian Shi
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
mouse	WB	Dev Cell; Regulation of reverse electron transfer at mitochondrial complex I by unconventional Notch action in cancer stem cells.; Authors - Rani Ojha
human	WB	Nat Commun; Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia.; Authors - Haiying Jiang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zhenminm (Verified Customer) (07-10-2019)

It is too much non-specific background.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: Mouse Testis

HEY1 Antibody Western Blot, validation (1:1,000 dilution) in Mouse Testis (Cat no:19929-1-AP)