HEY1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HEY1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

43

Rezensionen (1)

Kat-Nr. : 19929-1-AP

Synonyme

BHLHb31, CHF 2, CHF2, HERP2, HESR 1, HESR1, HEY1, hHRT1, HRT 1, HRT1, OAF1

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Galerie der Validierungsdaten

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  WB analysis using 19929-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19929-1-AP (HEY1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19929-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19929-1-AP (HEY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 19929-1-AP

  IP Result of anti-HEY1 (IP:19929-1-AP, 4ug; Detection:19929-1-AP 1:500) with mouse kidney tissue lysate 4400ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, A549-Zellen, NCI-H1299-Zellen, Rattenhodengewebe
• Erfolgreiche IP: Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 38 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 3 publications below

Produktinformation

19929-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, chIP, ELISA HEY1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HEY1 fusion protein Ag13906
• Vollständiger Name: hairy/enhancer-of-split related with YRPW motif 1
• Berechnetes Molekulargewicht: 304 aa, 33 kDa
• Beobachtetes Molekulargewicht: 32-34 kDa
• GenBank-Zugangsnummer: BC001873
• Gene symbol: HEY1
• Gene ID (NCBI): 23462
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hairy/enhancer of split-related proteins are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. And HEY1 is one of such protein. It is the direct downstream effector of Notch signaling which may be required for cardiovascular development. It preferentially bind to the canonical E box sequence 5'-CACGTG-3' and acts as a transcriptional repressor. HEY1 is a 33kDa protein and forms dimer by self-associating.

Publikationen

Species	Application	Title
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
mouse	WB	Dev Cell; Regulation of reverse electron transfer at mitochondrial complex I by unconventional Notch action in cancer stem cells.; Authors - Rani Ojha
human	WB	Nat Commun; Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia.; Authors - Haiying Jiang
human	WB	Theranostics; Exosome-mediated delivery of RBP-J decoy oligodeoxynucleotides ameliorates hepatic fibrosis in mice.; Authors - Fei He
human	WB	Oncogene; microRNA-23a, -27a and -24 synergistically regulate JAK1/Stat3 cascade and serve as novel therapeutic targets in human acute erythroid leukemia.; Authors - R Su
	WB	Cell Mol Biol Lett; Notch mediates the glycolytic switch via PI3K/Akt signaling to support embryonic development; Authors - Heng Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Zhenminm (Verified Customer) (07-10-2019)

It is too much non-specific background.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: Mouse Testis

HEY1 Antibody Western Blot, validation (1:1,000 dilution) in Mouse Testis (Cat no:19929-1-AP)