HEY2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HEY2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

42

Rezensionen (3)

Kat-Nr. : 10597-1-AP

Synonyme

bHLHb32, CHF1, GRIDLOCK, GRL, hCHF1, HERP, HERP1, HESR 2, HESR2, HEY2, hHRT2, HRT 2, HRT2, Protein gridlock homolog

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 10597-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10597-1-AP (HEY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10597-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10597-1-AP (HEY2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 10597-1-AP

  4T1 cells were subjected to SDS PAGE followed by western blot with 10597-1-AP (HEY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10597-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10597-1-AP (HEY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10597-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10597-1-AP (HEY2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 10597-1-AP

  IP Result of anti-HEY2 (IP:10597-1-AP, 5ug; Detection:10597-1-AP 1:500) with mouse heart tissue lysate 7500ug.

• 

  IHC staining of human prostate cancer using 10597-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10597-1-AP (HEY2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 10597-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10597-1-AP (HEY2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10597-1-AP

  Immunofluorescent analysis of HepG2 cells, using HEY2 antibody 10597-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, 4T1-Zellen, Mausherzgewebe, MCF-7-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 25 publications below
• IHC: See 9 publications below
• IF: See 8 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Produktinformation

10597-1-AP bindet in WB, IP, IHC, IF, CoIP, ChIP, ELISA HEY2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HEY2 fusion protein Ag0913
• Vollständiger Name: hairy/enhancer-of-split related with YRPW motif 2
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC007707
• Gene symbol: HEY2
• Gene ID (NCBI): 23493
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hairy/enhancer of split-related proteins, such as HEY2, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. HEY2 is similarly expressed in the somites whereas it shows a complementary expression in the heart, the craniofacial region and the nervous system. This antibody is a rabbit polyclonal antibody raised against full length of human HEY2

Publikationen

Species	Application	Title
human	IF	Cell; Multi-chamber cardioids unravel human heart development and cardiac defects; Authors - Clara Schmidt
human	IF	Cell Stem Cell; Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts.; Authors - Blair K Gage
mouse	WB	Nat Neurosci; Zeb2 recruits HDAC-NuRD to inhibit Notch and controls Schwann cell differentiation and remyelination.; Authors - Lai Man Natalie Wu
human	chIP	Nat Commun; Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis.; Authors - Jared M Churko
human	IF	Nat Commun; Generation of mature compact ventricular cardiomyocytes from human pluripotent stem cells.; Authors - Shunsuke Funakoshi
human	WB	Nat Commun; Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia.; Authors - Haiying Jiang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

CHAO (Verified Customer) (03-31-2022)

Weak band around correct size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart

FH

Tom (Verified Customer) (08-22-2020)

I performed western blot with mouse heart tissue lysate using Hey2 antibody. A specific ~36 kDa Hey2 band was observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart

FH

Tom (Verified Customer) (08-22-2020)

This Hey2 antibody is highly recommended for detecting Hey2 on frozen tissue sections! I used this Hey2 antibody for immunofluorescence (IF). It clearly had nuclear staining signal in compact myocardium of embryonic heart, which is consistent with Hey2's well-known expression pattern.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse embryonic heart

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