HGD Rekombinanter Antikörper

HGD Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242485E11

Kat-Nr. : 84931-5-PBS

Synonyme

242485E11, AKU, EC:1.13.11.5, HGO, Homogentisate 1,2 dioxygenase

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  WB analysis using 84931-5-RR (same clone as 84931-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84931-5-RR (HGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84931-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 84931-5-RR (same clone as 84931-5-PBS)

  rat liver tissue lysates were subjected to SDS PAGE followed by western blot with 84931-5-RR (HGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84931-5-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 84931-5-RR (same clone as 84931-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84931-5-RR (HGD antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84931-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84931-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84931-5-RR against Human HGD were performed. The affinity constant is 1.38 nM.

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Geprüfte Anwendungen

Produktinformation

84931-5-PBS bindet in WB, IHC, Indirect ELISA HGD und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HGD fusion protein Ag9544
• Vollständiger Name: homogentisate 1,2-dioxygenase (homogentisate oxidase)
• Berechnetes Molekulargewicht: 37 kDa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC020792
• Gene symbol: HGD
• Gene ID (NCBI): 3081
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Homogentisate1,2-dioxygenase (HGD), also named as HGO, is a mononuclear Fe(II)-dependent oxygenase that catalyzes the third step in the pathway for the catabolism of tyrosine, the conversion of homogentisate (HG) to maleylacetoacetate (MAA) and it can exsit as a dimer or trimer(PMID:14678794). HGD consists of a single type of subunit with no intermolecular disulfide bridges and requires Fe2+ as a cofactor(PMID: 7705358). Defects in HGD are the cause of alkaptonuria (AKU)(PMID:10594001).