HGF Polyklonaler Antikörper

HGF Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26881-1-PBS

Synonyme

HGF Alpha, Hepatocyte growth factor, Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain, Hepatopoietin-A

---

• 

  WB analysis using 26881-1-AP (same clone as 26881-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26881-1-AP (HGF alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 26881-1-AP (same clone as 26881-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26881-1-AP (HGF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 26881-1-AP (same clone as 26881-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26881-1-AP (HGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 26881-1-AP (same clone as 26881-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26881-1-AP (HGF alpha antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 26881-1-AP (same clone as 26881-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26881-1-AP (HGF alpha antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 26881-1-AP (same clone as 26881-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26881-1-AP (HGF Alpha antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 26881-1-AP (same clone as 26881-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26881-1-AP (HGF Alpha antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 26881-1-AP (same clone as 26881-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HGF Alpha antibody (26881-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26881-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

26881-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HGF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HGF fusion protein Ag25456
• Vollständiger Name: hepatocyte growth factor (hepapoietin A; scatter factor)
• Beobachtetes Molekulargewicht: 69 kDa
• GenBank-Zugangsnummer: BC130286
• Gene symbol: HGF
• Gene ID (NCBI): 3082
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hepatocyte growth factor (HGF) is the most potent mitogen of mature hepatocytes in primary culture. HGF is derived from a biologically inactive single chain precursor of 728 amino acids (pro-HGF) localized mostly on the cell surface and in the extracellular matrix. The mature form produced following proteolytic cleavage is composed of a 69-kDa α-subunit (containing four kringle domains) and the 34 kDa β-subunit, similar to the catalytic domain of serine proteases, but with amino acid substitutions in the active site. HGF is a pleiotropic cytokine which exerts a variety of effects on several cells, being involved in the regulation of many biological processes, such as inflammation, tissue repair, morphogenesis, angiogenesis, tumour propagation, immunomodulation of viral infections and cardio-metabolic activities.