HGS Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HGS Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 10390-1-AP

Synonyme

HGS, HRS, Protein pp110, ZFYVE8

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Galerie der Validierungsdaten

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  WB analysis using 10390-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10390-1-AP (HGS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10390-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10390-1-AP (HGS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10390-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10390-1-AP (HGS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10390-1-AP

  IP Result of anti-HGS (IP:10390-1-AP, 5ug; Detection:10390-1-AP 1:1000) with mouse brain tissue lysate 3000ug.

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  IHC staining of human liver using 10390-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10390-1-AP (HGS Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 10390-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10390-1-AP (HGS Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse brain using 10390-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10390-1-AP (HGS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10390-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10390-1-AP (HGS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10390-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HGS antibody (10390-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Produktinformation

10390-1-AP bindet in WB, IP, IHC, IF, ELISA HGS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HGS fusion protein Ag0589
• Vollständiger Name: hepatocyte growth factor-regulated tyrosine kinase substrate
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC003565
• Gene symbol: HGS
• Gene ID (NCBI): 9146
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS, synonyms: HRS, ZFYVE8) is a 110 to 115-kDa zinc finger phosphotyrosine protein inducible by stimulation with interleukin 2 (IL-2), granulocyte-macrophage colony-stimulating factor (GM-CSF) as well as hepatocyte growth factor (HGF), and is associated with signal-transducing adaptor molecule (STAM). HGS suppresses DNA synthesis upon stimulation with IL-2 and GM-CSF, counteracting the function of STAM which is critical for cell growth signaling mediated by the cytokines. HGS also interacts with the neurofibromatosis 2 tumor suppressor protein schwannomin/merlin. The growth suppression activity of schwannomin/merlin requires HGS and the binding of schwannomin/merlin to HGS facilitates its ability to function as a tumor suppressor, probably by inhibiting STAT activation.

Publikationen

Species	Application	Title
human	WB	Cell Res; RAB31 marks and controls an ESCRT-independent exosome pathway.; Authors - Denghui Wei
human	IHC	Sci Rep; β-defensin 1 expression in HCV infected liver/liver cancer: an important role in protecting HCV progression and liver cancer development.; Authors - Yue-Ming Ling
human	WB,IF	Mol Biol Cell; The ubiquitin ligase Deltex-3L regulates endosomal sorting of the G protein-coupled receptor CXCR4.; Authors - Justine Holleman
human	IF	J Cell Sci; Neutral sphingomyelinase 2 controls exosome secretion by counteracting V-ATPase-mediated endosome acidification.; Authors - Dolma Choezom
human	IF	iScience; VPS28 regulates brain vasculature by controlling neuronal VEGF trafficking through extracellular vesicle secretion.; Authors - Xiaohua Dong
human	IF	iScience; Compartmentalization of casein kinase 1 γ CSNK1G controls the intracellular trafficking of ceramide.; Authors - Asako Goto

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