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HGS Polyklonaler Antikörper

HGS Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Kat-Nr. : 10390-1-AP

Synonyme

ZFYVE8, Protein pp110, Hrs, Hepatocyte growth factor-regulated tyrosine kinase substrate



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, K-562-Zellen, Maushirngewebe, Rattenhirngewebe
Erfolgreiche IPMaushirngewebe
Erfolgreiche Detektion in IHChumanes Lebergewebe, Maushirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

10390-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA HGS und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HGS fusion protein Ag0589
Vollständiger Name hepatocyte growth factor-regulated tyrosine kinase substrate
Berechnetes Molekulargewicht 86 kDa
Beobachtetes Molekulargewicht 110 kDa
GenBank-ZugangsnummerBC003565
Gene symbol HGS
Gene ID (NCBI) 9146
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS, synonyms: HRS, ZFYVE8) is a 110 to 115-kDa zinc finger phosphotyrosine protein inducible by stimulation with interleukin 2 (IL-2), granulocyte-macrophage colony-stimulating factor (GM-CSF) as well as hepatocyte growth factor (HGF), and is associated with signal-transducing adaptor molecule (STAM). HGS suppresses DNA synthesis upon stimulation with IL-2 and GM-CSF, counteracting STAM's function, which is critical for cell growth signaling mediated by the cytokines. HGS also interacts with the neurofibromatosis 2 tumor suppressor protein Schwannomin/merlin. The growth suppression activity of schwannoma/merlin requires HGS. The binding of schwannoma/merlin to HGS facilitates its ability to function as a tumor suppressor, probably by inhibiting STAT activation.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for HGS antibody 10390-1-APProtokoll herunterladen
IHC protocol for HGS antibody 10390-1-APProtokoll herunterladenl
IF protocol for HGS antibody 10390-1-APProtokoll herunterladen
IP protocol for HGS antibody 10390-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell Res

RAB31 marks and controls an ESCRT-independent exosome pathway.

Authors - Denghui Wei
humanWB

EMBO Rep

The USP12/46 deubiquitinases protect integrins from ESCRT-mediated lysosomal degradation

Authors - Kaikai Yu
humanIHC

Sci Rep

β-defensin 1 expression in HCV infected liver/liver cancer: an important role in protecting HCV progression and liver cancer development.

Authors - Yue-Ming Ling
humanWB,IF

Mol Biol Cell

The ubiquitin ligase Deltex-3L regulates endosomal sorting of the G protein-coupled receptor CXCR4.

Authors - Justine Holleman
humanIF

J Cell Sci

Neutral sphingomyelinase 2 controls exosome secretion by counteracting V-ATPase-mediated endosome acidification.

Authors - Dolma Choezom
Human

J Virol

Human Cytomegalovirus Hijacks WD Repeat Domain 11 for Virion Assembly Compartment Formation and Virion Morphogenesis.

Authors - Bo Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Wojciech (Verified Customer) (09-10-2025)

Tested HG5 antibody in T-cells and it consistently detected the expected 100 kDa band with strong specificity and very low background. Results were reproducible across experiments, and overall performance was good.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: leukemic T-cells
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