HGS Monoklonaler Antikörper

HGS Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3B10D6

Kat-Nr. : CL647-67818

Synonyme

HGS, HRS, Protein pp110, ZFYVE8

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Galerie der Validierungsdaten

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  IF Staining of HepG2 using CL647-67818

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 647 HGS antibody (CL647-67818, Clone: 3B10D6 ) at dilution of 1:100, CoraLite®488 Beta Actin antibody (CL488-66009, Clone: 2D4H5, green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-67818 bindet in IF HGS und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HGS fusion protein Ag28610
• Vollständiger Name: hepatocyte growth factor-regulated tyrosine kinase substrate
• Berechnetes Molekulargewicht: 86 kDa
• GenBank-Zugangsnummer: BC003565
• Gene symbol: HGS
• Gene ID (NCBI): 9146
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS, synonyms: HRS, ZFYVE8) is a 110 to 115-kDa zinc finger phosphotyrosine protein inducible by stimulation with interleukin 2 (IL-2), granulocyte-macrophage colony-stimulating factor (GM-CSF) as well as hepatocyte growth factor (HGF), and is associated with signal-transducing adaptor molecule (STAM). HGS suppresses DNA synthesis upon stimulation with IL-2 and GM-CSF, counteracting the function of STAM which is critical for cell growth signaling mediated by the cytokines. HGS also interacts with the neurofibromatosis 2 tumor suppressor protein schwannomin/merlin. The growth suppression activity of schwannomin/merlin requires HGS and the binding of schwannomin/merlin to HGS facilitates its ability to function as a tumor suppressor, probably by inhibiting STAT activation.