HHIP Polyklonaler Antikörper

HHIP Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29466-1-PBS

Synonyme

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  WB analysis using 29466-1-AP (same clone as 29466-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29466-1-AP (HHIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 29466-1-AP (same clone as 29466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 29466-1-AP (same clone as 29466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 29466-1-AP (same clone as 29466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 29466-1-AP (same clone as 29466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 29466-1-AP (same clone as 29466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 29466-1-AP (same clone as 29466-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HHIP antibody (29466-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29466-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HHIP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HHIP fusion protein Ag30869
• Vollständiger Name: hedgehog interacting protein
• Berechnetes Molekulargewicht: 700 aa, 79 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC025311
• Gene symbol: HHIP
• Gene ID (NCBI): 64399
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The hedgehog (HH) proteins are evolutionarily conserved protein, which are important morphogens for a wide range of developmental processes, including anteroposterior patterns of limbs and regulation of left-right asymmetry in embryonic development. Multiple cell-surface receptors are responsible for transducing and/or regulating HH signals. HHIP is a highly conserved, vertebrate-specific inhibitor of HH signaling. It interacts with all three HH family members, SHH, IHH and DHH.