HIBADH Polyklonaler Antikörper

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• KD/KO Validated

HIBADH Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 13466-1-AP

Synonyme

HIBADH, NS5ATP1

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Galerie der Validierungsdaten

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  WB analysis using 13466-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13466-1-AP (HIBADH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 13466-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13466-1-AP (HIBADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 13466-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13466-1-AP (HIBADH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 13466-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13466-1-AP (HIBADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 13466-1-AP

  IP result of anti-HIBADH (IP:13466-1-AP, 4ug; Detection:13466-1-AP 1:800) with mouse heart tissue lysate 4000ug.

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  IHC staining of human liver cancer using 13466-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13466-1-AP (HIBADH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13466-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13466-1-AP (HIBADH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13466-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13466-1-AP (HIBADH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13466-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13466-1-AP (HIBADH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Maus-Eierstockgewebe, Mausherzgewebe, Maus-Thymusgewebe, Rattenherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

13466-1-AP bindet in WB, IP, IHC, ELISA HIBADH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HIBADH fusion protein Ag4258
• Vollständiger Name: 3-hydroxyisobutyrate dehydrogenase
• Berechnetes Molekulargewicht: 336 aa, 35 kDa
• Beobachtetes Molekulargewicht: 32-35 kDa
• GenBank-Zugangsnummer: BC032324
• Gene symbol: HIBADH
• Gene ID (NCBI): 11112
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Med; PMID: 26950361; Authors - Cholsoon Jang ; KD Validated
mouse	WB	PLoS One; Multifactorial Comparative Proteomic Study of Cytochrome P450 2E1 Function in Chronic Alcohol Administration.; Authors - Yuan Wang