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HIBCH Monoklonaler Antikörper

HIBCH Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B7B8

Kat-Nr. : 68157-1-PBS

Synonyme

HIBYL COA H, HIB-CoA hydrolase, HIB CoA hydrolase, EC:3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase

Filter:
  • Western Blot (WB) analysis of various lysates using HIBCH Monoclonal antibody (68157-1-Ig)

    WB analysis using 68157-1-Ig (same clone as 68157-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68157-1-Ig (HIBCH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68157-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using HIBCH Monoclonal antibody (68157-1-Ig)

    WB analysis of HepG2 using 68157-1-Ig (same clone as 68157-1-PBS)

    WB result of HIBCH antibody (68157-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIBCH transfected HepG2 cells. This data was developed using the same antibody clone with 68157-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HIBCH Monoclonal antibody (68157-1-Ig)

    IF Staining of HepG2 using 68157-1-Ig (same clone as 68157-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (68157-1-Ig, Clone: 1B7B8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68157-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68157-1-PBS bindet in WB, IF/ICC, Indirect ELISA HIBCH und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Kaninchen, Maus, Ratte
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen HIBCH fusion protein Ag6473
Vollständiger Name 3-hydroxyisobutyryl-Coenzyme A hydrolase
Berechnetes Molekulargewicht 43 kDa
Beobachtetes Molekulargewicht38 kDa
GenBank-ZugangsnummerBC067822
Gene symbol HIBCH
Gene ID (NCBI) 26275
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301).