HIC1 Polyklonaler Antikörper

HIC1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (1)

Kat-Nr. : 24949-1-AP

Synonyme

Zinc finger and BTB domain-containing protein 29, ZBTB29, hypermethylated in cancer 1, Hic-1, hic 1

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• 

  WB analysis of Jurkat using 24949-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24949-1-AP (HIC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 24949-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24949-1-AP (HIC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat lung using 24949-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 24949-1-AP (HIC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 24949-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 24949-1-AP (HIC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Rattenlungengewebe, Ratten-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

24949-1-AP bindet in WB, IHC, IF, ELISA HIC1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HIC1 fusion protein Ag21849
• Vollständiger Name: hypermethylated in cancer 1
• Berechnetes Molekulargewicht: 714 aa, 75 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC157027
• Gene symbol: HIC1
• Gene ID (NCBI): 3090
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC,IF	Adv Sci (Weinh); HIC1 suppresses Tumor Progression and Enhances CD8+ T Cells Infiltration Through Promoting GSDMD-induced Pyroptosis in Gastric Cancer; Authors - Mengjie Kang
human	WB	Front Cell Dev Biol; Integrated Analysis of Ferroptosis-Related Biomarker Signatures to Improve the Diagnosis and Prognosis Prediction of Ovarian Cancer.; Authors - Huan Wang
human	WB,IHC	Cancer Cell Int; ZBTB7A, a miR-144-3p targeted gene, accelerates bladder cancer progression via downregulating HIC1 expression.; Authors - Junqiang Liu
human	IHC	Front Immunol; Ferroptosis-related gene HIC1 in the prediction of the prognosis and immunotherapeutic efficacy with immunological activity; Authors - Yanlin Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shazia (Verified Customer) (08-25-2025)

I used it to stain fibroblasts and it worked amazingly.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/300
• Cell Tissue Type: Mice Embryo