HIC2 Polyklonaler Antikörper

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• KD/KO Validated

HIC2 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 22788-1-AP

Synonyme

Hic 2, Hic 3, HIC2, HRG22, hypermethylated in cancer 2, KIAA1020, ZBTB30

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  WB analysis of Jurkat using 22788-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22788-1-AP (HIC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 22788-1-AP

  IP result of anti-HIC2 (IP:22788-1-AP, 4ug; Detection:22788-1-AP 1:300) with Jurkat cells lysate 2400ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche IP: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

22788-1-AP bindet in WB, IP, ELISA HIC2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HIC2 fusion protein Ag18789
• Vollständiger Name: hypermethylated in cancer 2
• Berechnetes Molekulargewicht: 615 aa, 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC094787
• Gene symbol: HIC2
• Gene ID (NCBI): 23119
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIC2 (hypermethylated in cancer 2) is a transcription factor related to the tumour suppressor HIC1, required for normal cardiac development and lost in distal variants of 22q11 Deletion Syndrome (PMID: 29061339). HIC2 is also a POK family protein, and the amino acid sequence of its BTB/POZ and zinc-finger domains are similar to those of HIC1(PMID: 31127867). Studies have shown that HIC2 plays a role in suppressing fetal genes for normal development of the heart and circulatory system, in embryogenesis (PMID: 24748541).

Publikationen

Species	Application	Title
human	WB	Nat Genet; HIC2 controls developmental hemoglobin switching by repressing BCL11A transcription; Authors - Peng Huang ; KD Validated
human	WB	Blood; Let-7 miRNAs repress HIC2 to regulate BCL11A transcription and hemoglobin switching; Authors - Peng Huang
human	WB	Am J Cancer Res; Small extracellular vesicle-associated miR-6068 promotes aggressive phenotypes of prostate cancer through miR-6068/HIC2/SIRT1 axis; Authors - Mohamed S A Gaballah