HIC2 Rekombinanter Antikörper

HIC2 Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230177E8

Kat-Nr. : 83162-7-RR

Synonyme

Hic-3, Hic-2, HIC1-related gene on chromosome 22 protein, Hic 3, Hic 2

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  IF Staining of HepG2 using 83162-7-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIC2 antibody (83162-7-RR, Clone: 230177E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of U2OS using 83162-7-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HIC2 antibody (83162-7-RR, Clone: 230177E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 83162-7-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Hic2 Recombinant Antibody (83162-7-RR, Clone:230177E8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83162-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83162-7-RR against Human HIC2 were performed. The affinity constant is 0.527 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83162-7-RR bindet in IF/ICC, FC (Intra), ELISA HIC2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HIC2 fusion protein Ag18789
• Vollständiger Name: hypermethylated in cancer 2
• Berechnetes Molekulargewicht: 615 aa, 66 kDa
• GenBank-Zugangsnummer: BC094787
• Gene symbol: HIC2
• Gene ID (NCBI): 23119
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIC2 (hypermethylated in cancer 2) is a transcription factor related to the tumor suppressor HIC1, required for normal cardiac development and lost in distal variants of 22q11 Deletion Syndrome (PMID: 29061339). HIC2 is also a POK family protein, and the amino acid sequence of its BTB/POZ and zinc-finger domains are similar to those of HIC1(PMID: 31127867). Studies have shown that HIC2 plays a role in suppressing fetal genes for normal development of the heart and circulatory system, in embryogenesis (PMID: 24748541).