HIC5 Polyklonaler Antikörper

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HIC5 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10565-1-PBS

Synonyme

Androgen receptor-associated protein of 55 kDa, ARA55, Hic-5, Hydrogen peroxide-inducible clone 5 protein, TGFB1I1

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  WB analysis using 10565-1-AP (same clone as 10565-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10565-1-AP (HIC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10565-1-PBS in a different storage buffer formulation.

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  WB analysis using 10565-1-AP (same clone as 10565-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10565-1-AP (HIC5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10565-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 10565-1-AP (same clone as 10565-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10565-1-AP (HIC5 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10565-1-PBS in a different storage buffer formulation.

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  IP experiment of A431 using 10565-1-AP (same clone as 10565-1-PBS)

  IP result of anti-HIC5 (IP:10565-1-AP, 4ug; Detection:10565-1-AP 1:500) with A431 cells lysate 1520ug. This data was developed using the same antibody clone with 10565-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 10565-1-AP (same clone as 10565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10565-1-AP (HIC5 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10565-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 10565-1-AP (same clone as 10565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10565-1-AP (HIC5 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10565-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 10565-1-AP (same clone as 10565-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HIC5 antibody (10565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10565-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 10565-1-AP (same clone as 10565-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using HIC5 antibody (10565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10565-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10565-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA HIC5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HIC5 fusion protein Ag0868
• Vollständiger Name: transforming growth factor beta 1 induced transcript 1
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC001830
• Gene symbol: HIC5
• Gene ID (NCBI): 7041
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

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