HIF-1 alpha Monoklonaler Antikörper

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• KD/KO Validated

HIF-1 alpha Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H3C12

Kat-Nr. : 66730-1-PBS

Synonyme

HIF1A, 1H3C12, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1

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  WB analysis of HeLa using 66730-1-Ig (same clone as 66730-1-PBS)

  Untreated and cobalt chloride treated HeLa and HepG2 cells were subjected to SDS-PAGE followed by western blot with 66730-1-Ig (HIF1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with YY1 antibody as loading control. This data was developed using the same antibody clone with 66730-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66730-1-Ig (same clone as 66730-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66730-1-Ig (HIF-1 alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66730-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66730-1-Ig (same clone as 66730-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66730-1-Ig (HIF-1 alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66730-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66730-1-Ig (same clone as 66730-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66730-1-Ig (HIF-1 alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66730-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66730-1-Ig (same clone as 66730-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66730-1-Ig (HIF-1 alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66730-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66730-1-PBS bindet in WB, IHC, Indirect ELISA HIF-1 alpha und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HIF-1 alpha fusion protein Ag15198
• Vollständiger Name: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• Berechnetes Molekulargewicht: 826 aa, 93 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC012527
• Gene symbol: HIF1A
• Gene ID (NCBI): 3091
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .