HIF-1 alpha Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HIF-1 alpha Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ChIP, Cell treatment, ELISA

Konjugation

Unkonjugiert

Publikationen

510

Rezensionen (5)

Kat-Nr. : 20960-1-AP

Synonyme

ARNT interacting protein, bHLHe78, HIF 1 alpha, HIF 1alpha, HIF1, HIF-1, HIF1 ALPHA, HIF-1 alpha, HIF1A, HIF-1α, Member of PAS protein 1, MOP1, PASD8

---

Galerie der Validierungsdaten

• 

  WB analysis using 20960-1-AP

  Untreated and Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 20960-1-AP (HIF1a antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20960-1-AP

  WB result of HIF-1 alpha antibody (20960-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIF-1 alpha transfected HeLa cells. Sample 1: non-treated sh-Control transfected HeLa cells, Sample 2: Cobalt Chloride treated sh-Control transfected HeLa cells, Sample 3: Cobalt Chloride treated sh-HIF-1 alpha transfected HeLa cells.

• 

  WB analysis using 20960-1-AP

  Untreated and Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 20960-1-AP (HIF1a antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20960-1-AP

  Untreated and Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 20960-1-AP (HIF1a antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 20960-1-AP

  IP Result of anti-HIF1a (IP:20960-1-AP, 4ug; Detection:20960-1-AP 1:300) with HeLa cells lysate 4000ug.

• 

  IHC staining of human thyroid cancer using 20960-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 20960-1-AP (HIF1a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 20960-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 20960-1-AP (HIF1a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 20960-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20960-1-AP (HIF1A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 20960-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20960-1-AP (HIF1A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human thyroid cancer using 20960-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20960-1-AP (HIF1A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human thyroid cancer using 20960-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20960-1-AP (HIF1A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 20960-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20960-1-AP (HIF1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 20960-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20960-1-AP (HIF1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 20960-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using HIF-1 alpha antibody (20960-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 20960-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed Cobalt Chloride treated HepG2 cells using HIF1a antibody (20960-1-AP) at dilution of 1:1800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 20960-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed Cobalt Chloride treated HepG2 cells using HIF1a antibody (20960-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 20960-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using HIF-1 alpha antibody (20960-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 20960-1-AP

  1X10^6 HeLa cells were stained with 0.2ug HIF1a antibody (20960-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Cobaltchlorid behandelte HeLa-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Schilddrüsenkarzinomgewebe, humanes Herzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: mit Cobaltchlorid behandelte HeLa-Zellen, mit Cobaltchlorid behandelte HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 44 publications below
• WB: See 426 publications below
• IHC: See 98 publications below
• IF: See 68 publications below
• IP: See 11 publications below
• CoIP: See 10 publications below
• ChIP: See 13 publications below

Produktinformation

20960-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ChIP, Cell treatment, ELISA HIF-1 alpha und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HIF-1 alpha fusion protein Ag15198
• Vollständiger Name: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• Berechnetes Molekulargewicht: 826 aa, 93 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC012527
• Gene symbol: HIF1A
• Gene ID (NCBI): 3091
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .

Publikationen

Species	Application	Title
	WB	Gastroenterology; Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells; Authors - Decai Wang
human	WB	Cancer Cell; Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.; Authors - Ling Li
	IF	Cancer Cell; Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunity; Authors - Agnieszka Chryplewicz
		Nat Cell Biol; A single-cell transcriptomic landscape of the lungs of patients with COVID-19.; Authors - Si Wang
	WB	ACS Nano; Augmenting Intracellular Cargo Delivery of Extracellular Vesicles in Hypoxic Tissues through Inhibiting Hypoxia-Induced Endocytic Recycling; Authors - Bide Tong
human		Mol Cell; KDM3A Senses Oxygen Availability to Regulate PGC-1α-Mediated Mitochondrial Biogenesis.; Authors - Xu Qian

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Carlo (Verified Customer) (01-18-2023)

The quality of the band in western blot is well defined

• Applications: Western Blot
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Neuroblastoma cell line

FH

Davide (Verified Customer) (09-20-2022)

The image uploaded refers to a hypoxic condition O2<1%. The antibody signal was aspecific and generated aspecific binding in the middle of the organoid.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Cholangiocyte organoids

HIF-1 alpha Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocyte organoids (Cat no:20960-1-AP)

FH

Christian (Verified Customer) (01-07-2022)

Unfortunately much background an many unspecific bands in lysates of human chondrocytes and BV2 microclia cells (less unspecific bands in rat chondrocytes).

• Applications: Western Blot
• Cell Tissue Type: human chondrocytes

HIF-1 alpha Antibody Western Blot validation ( dilution) in human chondrocytes (Cat no:20960-1-AP)

FH

Diane (Verified Customer) (02-17-2021)

Separated on a 7.5% SDS PAGE gel, transferred to nitrocellulose membrane. Block with 5% dry milk/PBST at room temperature for one hour. 1:1000 dilution of the primary antibody in blocking solution. Incubated overnight at 4 degrees C on a rocker platform. Secondary antibody 1:1000 secondary (anti rabbit HRP) incubation for two hours at room temperature. Visualization with the Opti-4CN chromogenic substrate kit (BioRad).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: primary glioblastoma cell lysates

HIF-1 alpha Antibody Western Blot validation (1:1000 dilution) in primary glioblastoma cell lysates (Cat no:20960-1-AP)

FH

Chun (Verified Customer) (12-05-2019)

A good antibody

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HeLa cells

Verwandte Produkte

HIF-1 alpha Polyclonal antibody

20960-1-AP

VEGFA Polyclonal antibody

19003-1-AP

ARNT,HIF1B Polyclonal antibody

14105-1-AP

P53 Polyclonal antibody

10442-1-AP

PHD2/EGLN1 Polyclonal antibody

20368-1-AP

EPO Polyclonal antibody

17908-1-AP

LDHA-Specific Polyclonal antibody

19987-1-AP