HIF1AN Polyklonaler Antikörper

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• KD/KO Validated

HIF1AN Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 10646-1-AP

Synonyme

Factor inhibiting HIF 1, FIH 1, FIH1, HIF1AN

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  WB analysis of HEK-293 using 10646-1-AP

  WB result of HIF1AN antibody (10646-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIF1AN transfected HEK-293 cells.

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  WB analysis using 10646-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10646-1-AP (HIF1AN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10646-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10646-1-AP (HIF1AN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10646-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10646-1-AP (HIF1AN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

10646-1-AP bindet in WB, IHC, ELISA HIF1AN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HIF1AN fusion protein Ag0997
• Vollständiger Name: hypoxia inducible factor 1, alpha subunit inhibitor
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC007719
• Gene symbol: HIF1AN
• Gene ID (NCBI): 55662
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIF1AN (Hypoxia-inducible factor 1-alpha inhibitor) is also named FIH1. It is identified as a molecular oxygen-dependent dioxygenase that blunts the transcriptional activity of HIF and has also been implicated in HIF-dependent and -independent hypoxia responses(PMID:20720525). Structural studies indicate that an FIH-1 homodimer forms in solution and is essential for FIH-1 activity(PMID:16154531).

Publikationen

Species	Application	Title
mouse,rat	WB	Cell Death Dis; Asb10 accelerates pathological cardiac remodeling by stabilizing HSP70; Authors - Ke Lin
rat	WB	Bioact Mater; Development of a novel RNAi therapy: Engineered miR-31 exosomes promoted the healing of diabetic wounds.; Authors - Jinghuan Huang
human	WB	Am J Cancer Res; Experimental and clinical evidence suggests that GRPEL2 plays an oncogenic role in HCC development.; Authors - Ming-Chun Lai
rat	WB	Heliyon; LncRNA OIP5-AS1 regulates ferroptosis and mitochondrial dysfunction-mediated apoptosis in spinal cord injury by targeting the miR-128-3p/Nrf2 axis; Authors - Zhensong Jiang