HIF-1 alpha Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

HIF-1 alpha Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(72)

CloneNo.

1H3C12

Rezensionen (3)

Kat-Nr. : 66730-1-Ig

Synonyme

HIF1A, 1H3C12, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1

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• 

  WB analysis of HeLa using 66730-1-Ig

  Untreated and cobalt chloride treated HeLa and HepG2 cells were subjected to SDS-PAGE followed by western blot with 66730-1-Ig (HIF1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with YY1 antibody as loading control.

• 

  IHC staining of human ovary tumor using 66730-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66730-1-Ig (HIF-1 alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66730-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66730-1-Ig (HIF-1 alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66730-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66730-1-Ig (HIF-1 alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66730-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66730-1-Ig (HIF-1 alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, mit Cobaltchlorid behandelte HeLa-Zellen, mit Cobaltchlorid behandelte HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 61 publications below
• IHC: See 15 publications below
• IF: See 17 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

66730-1-Ig bindet in WB, IHC, IF, IP, CoIP, ChIP, ELISA HIF-1 alpha und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HIF-1 alpha fusion protein Ag15198
• Vollständiger Name: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• Berechnetes Molekulargewicht: 826 aa, 93 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC012527
• Gene symbol: HIF1A
• Gene ID (NCBI): 3091
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .

Publikationen

Species	Application	Title
human	WB,IP,IHC,IF	Adv Sci (Weinh); Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation; Authors - Huan Li
	IF	Cell Rep Med; Ultrasound-visible engineered bacteria for tumor chemo-immunotherapy; Authors - Yaozhang Yang
human	WB	J Immunother Cancer; TSH-TSHR axis promotes tumor immune evasion.; Authors - Zhenghao Wu
human	WB	Theranostics; ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.; Authors - Xiaoxin Zhang
	WB	Phytomedicine; mTOR/HIF-1α pathway-mediated glucose reprogramming and macrophage polarization by Sini decoction plus ginseng soup in ALF; Authors - Junli Zhang
human	WB	Oxid Med Cell Longev; Chitosan Oligosaccharides Alleviate H2O2-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway.; Authors - Ziwei Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

S (Verified Customer) (05-16-2024)

Excellent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: AC16

HIF-1 alpha Antibody Western Blot validation (1000 dilution) in AC16 (Cat no:66730-1-Ig)

FH

balawant (Verified Customer) (12-20-2022)

I have used this antibody for western blot and it is working great

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: renal Cancer cell line

FH

Diane (Verified Customer) (02-02-2021)

incubated over night 4 degrees C. 1:2500 HRP conjugated secondary. Opti-4CN substrate kit for detection. Also tried ICC on cytospins so ran out of trial size before fully optimized but has potential to provide the information that I am needing.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1250
• Cell Tissue Type: Cell lysates glioblastoma primary cells

HIF-1 alpha Antibody Western Blot validation (1:1250 dilution) in Cell lysates glioblastoma primary cells (Cat no:66730-1-Ig)