HIF2α/EPAS1 Monoklonaler Antikörper

HIF2α/EPAS1 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F6B12

Kat-Nr. : 66731-1-PBS

Synonyme

bHLHe73, ECYT4, EPAS 1, EPAS1, HIF 1 alpha like factor, HIF 2 alpha, HIF2 alpha, HIF2A, HIF-2A, HIF2α, HIF-2α, HLF, Member of PAS protein 2, MOP2, PASD2

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  WB analysis of HeLa using 66731-1-Ig (same clone as 66731-1-PBS)

  untreated and cobalt chloride treated Hela cells were subjected to SDS PAGE followed by western blot with 66731-1-Ig (EPAS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66731-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66731-1-Ig (same clone as 66731-1-PBS)

  untreated and cobalt chloride treated HepG2 cells were subjected to SDS PAGE followed by western blot with 66731-1-Ig (EPAS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66731-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66731-1-Ig (same clone as 66731-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66731-1-Ig (EPAS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66731-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66731-1-Ig (same clone as 66731-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66731-1-Ig (EPAS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66731-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66731-1-PBS bindet in WB, IHC, Indirect ELISA HIF2α/EPAS1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HIF2α/EPAS1 fusion protein Ag24886
• Vollständiger Name: endothelial PAS domain protein 1
• Berechnetes Molekulargewicht: 96 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC051338
• Gene symbol: HIF2α
• Gene ID (NCBI): 2034
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Endothelial PAS domain-containing protein 1 (EPAS1), also known as Hypoxia-inducible factor 2-alpha (HIF2-alpha,HIF2A), is a transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. EPAS1 is expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.