HIF3A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HIF3A Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 27650-1-AP

Synonyme

HIF3 alpha, HIF 3A4, HIF 3A2, HIF 3A, HIF 3 alpha

---

• 

  WB analysis of A431 using 27650-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27650-1-AP

  Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27650-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 27650-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using HIF3A antibody (27650-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Cobaltchlorid behandelte HeLa-Zellen, A431-Zellen
• Erfolgreiche Detektion in IF/ICC: mit Tunicamycin behandelte HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 3 publications below

Produktinformation

27650-1-AP bindet in WB, IHC, IF/ICC, ELISA HIF3A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HIF3A fusion protein Ag26574
• Vollständiger Name: hypoxia inducible factor 3, alpha subunit
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: NM_022462
• Gene symbol: HIF3A
• Gene ID (NCBI): 64344
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIF3A, Hypoxia-inducible factor 3-alpha, acts as a transcriptional regulator in adaptive response to low oxygen tension (PMID:11573933, PMID:16126907, PMID:19694616, PMID:20416395, PMID:21069422). It functions as an inhibitor of angiogenesis in hypoxic cells of the cornea. HIF3A also plays a role in the development of the cardiorespiratory system. HIF3A was up-regulated by hypoxia (PMID:16775626). Multiple alternatively spliced transcript variants have been found.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Identification of oleoylethanolamide as an endogenous ligand for HIF-3α.; Authors - Xiaotong Diao
human	WB	Mol Metab; Genome-wide association study of adipocyte lipolysis in the GENetics of adipocyte lipolysis (GENiAL) cohort.; Authors - Agné Kulyté ; KD Validated
mouse	WB	Front Cell Dev Biol; HIF3A Inhibition Triggers Browning of White Adipocytes via Metabolic Rewiring.; Authors - Francesca Cuomo ; KD Validated
mouse	WB	Toxicol Lett; Knockdown of long noncoding RNA MIAT attenuates cigarette smoke-induced airway remodeling by downregulating miR-29c-3p-HIF3A axis.; Authors - Wenchao Gu
human	IHC	ERJ Open Res; Dynamic analysis of gene signatures in the progression of COPD; Authors - Junchao Jiang
mouse	IHC	Bioact Mater; Synergistic rheumatoid arthritis therapy by interrupting the detrimental feedback loop to orchestrate hypoxia M1 macrophage polarization using an enzyme-catalyzed nanoplatform; Authors - Dong Guo