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HIG2 Polyklonaler Antikörper

HIG2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15587-1-PBS

Synonyme

C7orf68, HIG 2, HIG-2, HILPDA, Hypoxia-inducible gene 2 protein

Filter:
  • Western Blot (WB) analysis of various lysates using HIG2 Polyclonal antibody (15587-1-AP)

    WB analysis using 15587-1-AP (same clone as 15587-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15587-1-AP (HIG2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using HIG2 Polyclonal antibody (15587-1-AP)

    IHC staining of mouse kidney using 15587-1-AP (same clone as 15587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15587-1-AP (HIG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15587-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using HIG2 Polyclonal antibody (15587-1-AP)

    IF Staining of A549 using 15587-1-AP (same clone as 15587-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HIG2 antibody (15587-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15587-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

15587-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HIG2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HIG2 fusion protein Ag7829
Vollständiger Name chromosome 7 open reading frame 68
Berechnetes Molekulargewicht 7 kDa
Beobachtetes Molekulargewicht 7-10 kDa
GenBank-ZugangsnummerBC001863
Gene symbol C7orf68
Gene ID (NCBI) 29923
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIG2 (Hypoxia inducible gene 2), also known as hypoxia inducible lipid droplet associated (HILPDA), is a gene that plays a significant role in the context of hypoxia, particularly in cancer biology. HIG2 is can be induced by hypoxia and glucose deprivation. It is expressed under low-oxygen conditions, which are common in the tumor microenvironment, and has been identified as a target gene of hypoxia-inducible factor-1 (HIF-1). HIG2 is implicated in the development and progression of various types of cancer, including renal cell carcinoma(PMID: 15930302), cell lymphoma(PMID: 26757780), epithelial ovarian cancer(PMID: 20134266), and uterine cancer(PMID: 21614900). Its expression is often elevated in these cancers and is associated with poor prognosis.