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HIGD1A Polyklonaler Antikörper

HIGD1A Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21749-1-PBS

Synonyme

HIG1, HIG1 domain family member 1A, HIG1 domain family member 1A, mitochondrial, HSPC010, Hypoxia-inducible gene 1 protein

Filter:
  • Western Blot (WB) analysis of various lysates using HIGD1A Polyclonal antibody (21749-1-AP)

    WB analysis using 21749-1-AP (same clone as 21749-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21749-1-AP (HIGD1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21749-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HIGD1A Polyclonal antibody (21749-1-AP)

    WB analysis of HeLa using 21749-1-AP (same clone as 21749-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 21749-1-AP (HIGD1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21749-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HIGD1A Polyclonal antibody (21749-1-AP)

    IF Staining of HepG2 using 21749-1-AP (same clone as 21749-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIGD1A antibody (21749-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21749-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

21749-1-PBS bindet in WB, IF/ICC, Indirect ELISA HIGD1A und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HIGD1A fusion protein Ag14027
Vollständiger Name HIG1 hypoxia inducible domain family, member 1A
Berechnetes Molekulargewicht 93 aa, 10 kDa
Beobachtetes Molekulargewicht 10 kDa
GenBank-ZugangsnummerBC000601
Gene symbol HIGD1A
Gene ID (NCBI) 25994
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (22342701). It is induced by hypoxia induction.This antibody is a rabbit polyclonal antibody raised against a full-length human HIGD1A protein.