HIGD1A Monoklonaler Antikörper

HIGD1A Monoklonal Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3A8E2

Kat-Nr. : 68231-1-PBS

Synonyme

RCF1 homolog A, Hypoxia-inducible gene 1 protein, HSPC010, HIG1 domain family member 1A, mitochondrial, HIG1 domain family member 1A

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  WB analysis using 68231-1-Ig (same clone as 68231-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68231-1-Ig (HIGD1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68231-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 68231-1-Ig (same clone as 68231-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HIGD1A antibody (68231-1-Ig, Clone: 3A8E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68231-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50454-2

  Cytometric bead array standard curve of MP50454-2, HIGD1A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68231-1-PBS. Detection antibody: 68231-4-PBS. Standard:Ag14027. Range: 0.391-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

68231-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA HIGD1A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HIGD1A fusion protein Ag14027
• Vollständiger Name: HIG1 hypoxia inducible domain family, member 1A
• Berechnetes Molekulargewicht: 93 aa, 10 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC000601
• Gene symbol: HIGD1A
• Gene ID (NCBI): 25994
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (PMID:22342701). It is induced by hypoxia induction.