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HIGD2A Polyklonaler Antikörper

HIGD2A Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21415-1-PBS

Synonyme

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  • Western Blot (WB) analysis of mouse kidney tissue using HIGD2A Polyclonal antibody (21415-1-AP)

    WB analysis of mouse kidney using 21415-1-AP (same clone as 21415-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21415-1-AP (HIGD2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21415-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using HIGD2A Polyclonal antibody (21415-1-AP)

    IF Staining of MCF-7 using 21415-1-AP (same clone as 21415-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HIGD2A antibody (21415-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21415-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

21415-1-PBS bindet in WB, IF/ICC, Indirect ELISA HIGD2A und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HIGD2A fusion protein Ag15892
Vollständiger Name HIG1 hypoxia inducible domain family, member 2A
Berechnetes Molekulargewicht 106 aa, 12 kDa
Beobachtetes Molekulargewicht11 kDa, 50 kDa
GenBank-ZugangsnummerBC007502
Gene symbol HIGD2A
Gene ID (NCBI) 192286
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mammalian HIGD-family proteins include two subgroups of yeast Rcf1 homologs, HIGD1A/B/C and HIGD2A/B. HIGD1A and HIGD2A (of 10.4 and 11.5 kDa, respectively) have two hydrophilic transmembrane domains and are localized in the inner mitochondrial membrane. HIGD1A and HIGD2A expression is induced under stress conditions like hypoxia or glucose deprivation in human cervical epithelial cells and murine cerebral cortical neurons. HIGD1A may play pleiotropic roles in mitochondrial respiratory chain biogenesis. HIGD2A forms a 50 kDa regulatory module with COX4-1, COX5B, and COX6A1, which binds to newly synthesized COX3 to promote its binding to the previously assembled COX1 + COX2 module (PMID:33291261,32375044).