HINFP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HINFP Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10066-2-PBS

Synonyme

---

• 

  WB analysis of human liver using 10066-2-AP (same clone as 10066-2-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 10066-2-AP (same clone as 10066-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 10066-2-AP (same clone as 10066-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

• 

  IP experiment of L02 using 10066-2-AP (same clone as 10066-2-PBS)

  IP result of anti-HINFP (IP:10066-2-AP, 4ug; Detection:10066-2-AP 1:2000) with L02 cells lysate 1040 ug. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

• 

  IP experiment of L02 using 10066-2-AP (same clone as 10066-2-PBS)

  IP result of anti-HINFP (IP:10066-2-AP, 4ug; Detection:10066-2-AP 1:2000) with L02 cells lysate 1040 ug. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 10066-2-AP (same clone as 10066-2-PBS)

  Immunofluorescent analysis of fixed Hela cells using 10066-2-AP (HINFP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

• 

  IF Staining of L02 using 10066-2-AP (same clone as 10066-2-PBS)

  Immunofluorescent analysis of L02 cells using 10066-2-AP (HINFP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

10066-2-PBS bindet in WB, IF/ICC, IP, Indirect ELISA HINFP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HINFP fusion protein Ag0115
• Vollständiger Name: histone H4 transcription factor
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC001073
• Gene symbol: HINFP
• Gene ID (NCBI): 25988
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Methylation of CpG dinucleotides is the major epigenetic modification in higher eukaryotic genomes. MIZF interacts with MBD2, a methyl-CpG-binding protein playing a critical role in DNA methylation-mediated transcriptional repression. MIZF functions as a negative regulator of transcription by binding to MBD2 and recruiting histone deacetylase (HDAC)-containing complexes. MIZF mRNA is present in various human tissues and cell lines and expressed as a 60kd protein.

{{ptg:RelatedPrimaryAntibodies}}