HINT1 Polyklonaler Antikörper

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• KD/KO Validated

HINT1 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 10717-1-AP

Synonyme

HINT, HINT1, PKCI 1, PKCI1, PRKCNH1, Protein kinase C inhibitor 1

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Galerie der Validierungsdaten

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  WB analysis using 10717-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10717-1-AP (HINT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat thymus using 10717-1-AP

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 10717-1-AP (HINT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 10717-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10717-1-AP (HINT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10717-1-AP

  IP Result of anti-HINT1 (IP:10717-1-AP, 3ug; Detection:10717-1-AP 1:500) with mouse brain tissue lysate 7500ug.

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  IHC staining of human colon cancer using 10717-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10717-1-AP (HINT1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10717-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10717-1-AP (HINT1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10717-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10717-1-AP (HINT1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10717-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10717-1-AP (HINT1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen, Raji-Zellen, Rattenhirngewebe, Ratten-Thymusgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Produktinformation

10717-1-AP bindet in WB, IP, IHC, IF, ELISA HINT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HINT1 fusion protein Ag1142
• Vollständiger Name: histidine triad nucleotide binding protein 1
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC007090
• Gene symbol: HINT1
• Gene ID (NCBI): 3094
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HINT1(Histidine triad nucleotide-binding protein 1) is also named as HINT, PKCI1, PRKCNH1,which is a member of the histidine triad (HIT) family, highly conserved in diverse species and ubiquitously expressed in mammalian tissues.It hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.This antibody is specific to HINT1.

Publikationen

Species	Application	Title
human	IF	Nat Commun; Second messenger Ap4A polymerizes target protein HINT1 to transduce signals in FcεRI-activated mast cells.; Authors - Jing Yu
		Oncogene; Post-translational modification of HINT1 mediates activation of MITF transcriptional activity in human melanoma cells.; Authors - A Motzik
human	WB	J Med Chem; A Chemical Strategy for Intracellular Arming of an Endogenous Broad-Spectrum Antiviral Nucleotide; Authors - Kellan T. Passow
human	IHC	Cell Cycle; The tumor suppressor HINT1 regulates MITF and β-catenin transcriptional activity in melanoma cells.; Authors - Genovese Giannicola G
human,mouse	WB	Pharmacogenomics J; Association of the histidine-triad nucleotide-binding protein-1 (HINT1) gene variants with nicotine dependence.; Authors - Jackson K J KJ
mouse	WB,IHC	J Proteome Res; Novel breast cancer metastasis-associated proteins.; Authors - Ho Jiapei J

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