HIP1 Polyklonaler Antikörper

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• KD/KO Validated

HIP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22231-1-PBS

Synonyme

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  WB analysis using 22231-1-AP (same clone as 22231-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 22231-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 22231-1-AP (same clone as 22231-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 22231-1-AP (same clone as 22231-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 22231-1-AP (same clone as 22231-1-PBS)

  IP result of anti-HIP1 (IP:22231-1-AP, 4ug; Detection:22231-1-AP 1:1000) with HeLa cells lysate 2500ug. This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 22231-1-AP (same clone as 22231-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HIP1 antibody (22231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

22231-1-PBS bindet in WB, IHC, IF/ICC, IP, ELISA HIP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HIP1 fusion protein Ag17576
• Vollständiger Name: huntingtin interacting protein 1
• Berechnetes Molekulargewicht: 1037 aa, 116 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: BC110545
• Gene symbol: HIP1
• Gene ID (NCBI): 3092
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.