HIP1 Monoklonaler Antikörper

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HIP1 Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3D3G3

Kat-Nr. : 67163-1-PBS

Synonyme

HIP 1, HIP I, HIP1, ILWEQ

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  WB analysis using 67163-1-Ig (same clone as 67163-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67163-1-Ig (same clone as 67163-1-PBS)

  WB result of HIP1 antibody (67163-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIP1 transfected HeLa cells. This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 67163-1-Ig (same clone as 67163-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 67163-1-Ig (same clone as 67163-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67163-1-Ig (same clone as 67163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67163-1-Ig (same clone as 67163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67163-1-Ig (same clone as 67163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 67163-1-Ig (same clone as 67163-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using HIP1 antibody (67163-1-Ig, Clone: 3D3G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67163-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA HIP1 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HIP1 fusion protein Ag17982
• Vollständiger Name: huntingtin interacting protein 1
• Berechnetes Molekulargewicht: 1037 aa, 116 kDa
• Beobachtetes Molekulargewicht: 110-120 kDa
• GenBank-Zugangsnummer: BC110545
• Gene symbol: HIP1
• Gene ID (NCBI): 3092
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIP1 (huntingtin interacting protein-1) is a endocytic protein that plays a role in clathrin-mediated endocytosis and trafficking. HIP1 can interact with huntingtin (HTT) protein whose mutations cause Huntington's disease (HD), thus gets involved in neurodegeneration. By stabilizing receptor tyrosine kinases following ligand-induced endocytosis, HIP1 may act to promote cell survival. HIP1 is overexpressed in a variety of human cancers and may be a potential predictor for cancer progression and prognosis.