• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

HIRA Rekombinanter Antikörper

HIRA Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240006E5

Kat-Nr. : 83194-5-PBS

Synonyme

Protein HIRA, HIRA/HIR, HIR, 240006E5

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HIRA Recombinant antibody (83194-5-RR)

    IF Staining of HeLa using 83194-5-RR (same clone as 83194-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIRA antibody (83194-5-RR, Clone: 240006E5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83194-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HIRA Recombinant antibody (83194-5-RR)

    IF Staining of HeLa using 83194-5-RR (same clone as 83194-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIRA antibody (83194-5-RR, Clone: 240006E5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83194-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using HIRA Recombinant antibody (83194-5-RR)

    FC experiment of K-562 using 83194-5-RR (same clone as 83194-5-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug HIRA Recombinant Antibody (83194-5-RR, Clone:240006E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83194-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83194-5-RR

    Affinity and Kinetic Characterization of 83194-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83194-5-RR against Human HIRA were performed. The affinity constant is 7.91 nM.

Geprüfte Anwendungen

Produktinformation

83194-5-PBS bindet in IF/ICC, FC (Intra), ELISA HIRA und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen HIRA fusion protein Ag34439
Vollständiger Name HIR histone cell cycle regulation defective homolog A (S. cerevisiae)
Berechnetes Molekulargewicht 112 kDa
GenBank-ZugangsnummerNM_003325
Gene symbol HIRA
Gene ID (NCBI) 7290
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.