Histone H1 Polyklonaler Antikörper

Histone H1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 18201-1-AP

Synonyme

HIST1H1A, Histone, HIST1, H1F1, H1-1

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  WB analysis of mouse thymus using 18201-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18201-1-AP (HIST1H1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 18201-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18201-1-AP (Histone H1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 18201-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18201-1-AP (Histone H1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 18201-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18201-1-AP (Histone H1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 18201-1-AP

  IP result of anti-Histone H1 (IP:18201-1-AP, 4ug; Detection:18201-1-AP 1:500) with mouse kidney tissue lysate 4400ug.

• 

  IHC staining of human stomach using 18201-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18201-1-AP (Histone H1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Thymusgewebe, Mausnierengewebe, Mauslungengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

18201-1-AP bindet in WB, IHC, IF, IP, ELISA Histone H1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Histone H1 fusion protein Ag12704
• Vollständiger Name: histone cluster 1, H1a
• Berechnetes Molekulargewicht: 215 aa, 22 kDa
• Beobachtetes Molekulargewicht: 28 kDa, 30 kDa
• GenBank-Zugangsnummer: BC101593
• Gene symbol: Histone H1
• Gene ID (NCBI): 3024
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Cell Calcium; IN BRAIN POST-ISCHEMIC PLASTICITY, Na+/Ca2+ EXCHANGER 1 AND Ascl1 INTERVENE IN MICROGLIA-DEPENDENT CONVERSION OF ASTROCYTES INTO NEURONAL LINEAGE.; Authors - Antonella Casamassa
mouse	WB,IP	Int J Mol Sci; The KDET Motif in the Intracellular Domain of the Cell Adhesion Molecule L1 Interacts with Several Nuclear, Cytoplasmic, and Mitochondrial Proteins Essential for Neuronal Functions; Authors - Ralf Kleene
mouse	IF	Int J Mol Sci; The Interactions of the 70 kDa Fragment of Cell Adhesion Molecule L1 with Topoisomerase 1, Peroxisome Proliferator-Activated Receptor γ and NADH Dehydrogenase (Ubiquinone) Flavoprotein 2 Are Involved in Gene Expression and Neuronal L1-Dependent Functions; Authors - Gabriele Loers
	WB	Pharmacol Res; YAP K236 acetylation facilitates its nucleic export and deprived the protection against cardiac hypertrophy in mice; Authors - Panxia Wang