Histone H1.5 Polyklonaler Antikörper

Histone H1.5 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18093-1-PBS

Synonyme

HIST1H1B, histone cluster 1, H1b, Histone, H1F5, H1-5

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  WB analysis using 18093-1-AP (same clone as 18093-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18093-1-AP (Histone H1.5 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 18093-1-AP (same clone as 18093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 18093-1-AP (same clone as 18093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 18093-1-AP (same clone as 18093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 18093-1-AP (same clone as 18093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 18093-1-AP (same clone as 18093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 18093-1-AP (same clone as 18093-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Histone H1.5 antibody (18093-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18093-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Histone H1.5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Histone H1.5 fusion protein Ag12818
• Vollständiger Name: histone cluster 1, H1b
• Berechnetes Molekulargewicht: 226 aa, 23 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC101581
• Gene symbol: HIST1H1B
• Gene ID (NCBI): 3009
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Histone H1.5, also known as Histone Cluster 1 H1 Family Member B (HIST1H1B), is a member of the Histone H1/H5 protein family. Histone H1.5 gene has been found to be frequently mutated in colorectal cancer and follicular lymphomas. HIST1H1B overexpression contributes to tumor aggressiveness. Histone H1.5 expression is upregulated in breast cancer and predicts poor prognosis(PMID: 34746019).