Acetyl-Histone H2A (Lys9) Rekombinanter Antikörper

Acetyl-Histone H2A (Lys9) Rekombinant Antikörper für WB, Dot Blot, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Dot Blot, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

1O17

Kat-Nr. : 82823-2-PBS

Synonyme

Ac Histone H2A, Ac Histone H2A (Lys9), Ac Histone H2A Lys9, Ac Histone H2A-Lys9, Acetyl Histone

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  WB analysis using 82823-2-RR (same clone as 82823-2-PBS)

  Trichostain A treated and untreated HeLa cell lysates were subjected to SDS PAGE followed by western blot with 82823-2-RR (Acetyl-Histone H2A (Lys9) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control. This data was developed using the same antibody clone with 82823-2-PBS in a different storage buffer formulation.

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  Dot Blot experiment of peptide using 82823-2-RR (same clone as 82823-2-PBS)

  Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2A (Lys9) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. Column 1: H2AK9ac. Column 2: Unmodified H2AK9. Column 3: H2AK5ac. Column 4: Unmodified H2AK5. Column 5: H2AK13Ac. Column 6: Unmodified H2AK13. Column 7: H2AK15ac. Column 8:Unmodified H2AK15. Column 9: Blank(PBS). This data was developed using the same antibody clone with 82823-2-PBS in a different storage buffer formulation.

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  ChIP experiment of HeLa using 82823-2-RR (same clone as 82823-2-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H2A (Lys9) (82823-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82823-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82823-2-PBS bindet in WB, Dot Blot, Indirect ELISA, ChIP-qPCR Acetyl-Histone H2A (Lys9) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 1, H2aa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC062211
• Gene symbol: HIST1H2AA
• Gene ID (NCBI): 221613
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.