Mono-Methyl-Histone H3 (Lys9) Rekombinanter Antikörper

Mono-Methyl-Histone H3 (Lys9) Rekombinant Antikörper für WB, Dot Blot, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, Dot Blot, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

3C23

Kat-Nr. : 82821-6-PBS

Synonyme

dmk9, H3 Me Lys9, H3 MeK9, H3 Methyl K9, H3 Methyl Lys9

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  WB analysis using 82821-6-RR (same clone as 82821-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82821-6-RR (Mono-Methyl-Histone H3 (Lys9) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82821-6-PBS in a different storage buffer formulation.

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  Dot Blot experiment of peptide using 82821-6-RR (same clone as 82821-6-PBS)

  Dot blot analysis was used to confirm the specificity of 82821-6-RR Mono-Methyl-Histone H3 (Lys9) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 82821-6-PBS in a different storage buffer formulation.

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  ChIP experiment of HeLa using 82821-6-RR (same clone as 82821-6-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Mono-Methyl-Histone H3 (Lys9) (82821-6-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82821-6-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82821-6-PBS bindet in WB, Dot Blot, Indirect ELISA, ChIP-qPCR Mono-Methyl-Histone H3 (Lys9) und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 1, H3a
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC066245
• Gene symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified. Methylation of Histone H3 at lysine 9 is linked to transcriptional repression.