Acetyl-Histone H3 (Lys18) Rekombinanter Antikörper

Acetyl-Histone H3 (Lys18) Rekombinant Antikörper für WB, ChIP, Dot Blot, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ChIP, Dot Blot, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

2E3

Kat-Nr. : 82832-1-PBS

Synonyme

Ac Histone H3, Ac Histone H3 (Lys18), Ac Histone H3 Lys18, Ac Histone H3-Lys18, Acetyl Histone

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  WB analysis of NIH/3T3 using 82832-1-RR (same clone as 82832-1-PBS)

  Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82832-1-RR (Acetyl-Histone H3 (Lys18) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control. This data was developed using the same antibody clone with 82832-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 82832-1-RR (same clone as 82832-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 82832-1-RR (Acetyl-Histone H3 (Lys18) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82832-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 82832-1-RR (same clone as 82832-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82832-1-RR (Acetyl-Histone H3 (Lys18) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82832-1-PBS in a different storage buffer formulation.

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  Dot Blot experiment of peptide using 82832-1-RR (same clone as 82832-1-PBS)

  Dot blot analysis was used to confirm the specificity of 82832-1-RR Acetyl-Histone H3 (Lys18) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. Column 1: H3AK18Ac. Column 2: Unmodified H3AK18. Column 3: H3AK36Ac. Column 4: Unmodified H3AK36. Column 5: H3AK23Ac. Column 6: Unmodified H3AK23. Column 7: H3AK122Ac. Column 8:Unmodified H3AK122. Column 9: H3AK27Ac. Column 10: Unmodified H3AK27. Column 11: H3AK14Ac. Column 12: Unmodified H3AK14. Column 13: H3AK56Ac. Column 14:Unmodified H3AK56. Column 15:H3AK9Ac. Column 16:Unmodified H3AK9. Column 17:H3AK4Ac. Column 18:Unmodified H3AK4. Column 19:Blank(PBS). This data was developed using the same antibody clone with 82832-1-PBS in a different storage buffer formulation.

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  ChIP experiment of HeLa using 82832-1-RR (same clone as 82832-1-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys18) (82832-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82832-1-PBS in a different storage buffer formulation.

• 

  ChIP experiment of HeLa using 82832-1-RR (same clone as 82832-1-PBS)

  Chromatin was prepared from HeLa cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys18) (82832-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82832-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82832-1-PBS bindet in WB, ChIP, Dot Blot, Indirect ELISA, ChIP-qPCR Acetyl-Histone H3 (Lys18) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 1, H3a
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC066245
• Gene symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.