Histone H4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Histone H4 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(72)

Rezensionen (1)

Kat-Nr. : 16047-1-AP

Synonyme

HIST1H4E, HIST1H4A, H4C5, H4C4, H4C3

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  WB analysis using 16047-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16047-1-AP

  WB result of Histone H4 antibody (16047-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Histone H4 transfected HEK-293 cells.

• 

  WB analysis of MCF-7 using 16047-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (HIST1H4E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16047-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (HIST1H4E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16047-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (HIST1H4E antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16047-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16047-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16047-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16047-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 16047-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (HIST1H4E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat thymus using 16047-1-AP

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16047-1-AP

  IP result of anti-Histone H4 (IP:16047-1-AP, 3ug; Detection:16047-1-AP 1:600) with HeLa cells lysate 2000ug.

• 

  IP experiment of HeLa using 16047-1-AP

  IP result of anti-Histone H4 (IP:16047-1-AP, 4ug; Detection:16047-1-AP 1:300) with HeLa cells lysate 2680ug.

• 

  IHC staining of mouse small intestine using 16047-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 16047-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 16047-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16047-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16047-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 16047-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16047-1-AP

  Immunofluorescent analysis of HeLa cells using 16047-1-AP (Histone H4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 16047-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Histone H4 antibody (16047-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 16047-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Histone H4 antibody (16047-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 16047-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Histone H4 (16047-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  ChIP experiment of HEK-293 using 16047-1-AP

  Chromatin was prepared from HEK-293 cells. The ChIP was performed with 10 µg of cross-linked chromatin, 5 µg of 16047-1-AP or Normal Rabbit IgG, and 60µl of Protein A sepharose beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, HT-1080.Zellen, MCF-7-Zellen, Mausnierengewebe, Maus-Thymusgewebe, Ratten-Thymusgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maus-Dünndarmgewebe, humanes Mammakarzinomgewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen
• Erfolgreiche ChIP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• CHImmunpräzipitation (IP): CHIP :

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 63 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below
• RIP: See 1 publications below

Produktinformation

16047-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA Histone H4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hefe, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Histone H4 fusion protein Ag8999
• Vollständiger Name: histone cluster 1, H4e
• Berechnetes Molekulargewicht: 102 aa, 11 kDa
• Beobachtetes Molekulargewicht: 14 kDa, 11 kDa
• GenBank-Zugangsnummer: BC012587
• Gene symbol: Histone H4
• Gene ID (NCBI): 8367
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Publikationen

Species	Application	Title
mouse	WB	Cell; Insights into Nucleosome Organization in Mouse Embryonic Stem Cells through Chemical Mapping.; Authors - Lilien N Voong ; KD Validated
human	WB	Cell Res; Mutual dependency between lncRNA LETN and protein NPM1 in controlling the nucleolar structure and functions sustaining cell proliferation.; Authors - Xianteng Wang
mouse	WB	Cell Res; SUMO suppresses and MYC amplifies transcription globally by regulating CDK9 sumoylation.; Authors - Fang Yu
		Mol Cell; An oncogenic JMJD6-DGAT1 axis tunes the epigenetic regulation of lipid droplet formation in clear cell renal cell carcinoma.; Authors - Jin Zhou
rat	WB	Nat Commun; Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.; Authors - Megha Jhanji
human	WB	Autophagy; The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.; Authors - Liangde Zheng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lucia (Verified Customer) (07-24-2025)

The antibody works well for CHIP experiments (3 ug per experiments) and acceptable for Western Blot (1:2000 dilution).

• Applications: Western Blot, ChIP