Histone H2B Polyklonaler Antikörper

Histone H2B Polyklonal Antikörper für WB, IF/ICC, ChIP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ChIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27740-1-AP

Synonyme

H2B3B, HIST3H2BB, H2B-clustered histone 26, H2BC26, H2B.U histone 1

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  WB analysis using 27740-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 27740-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 27740-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 27740-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using Histone H2B antibody (27740-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  ChIP experiment of HEK-293 using 27740-1-AP

  Chromatin was prepared from HEK-293 cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 18 µg of cross-linked chromatin, 5 µg of Histone H2B Antibody (27740-1-AP) or 5 ug of Normal Rabbit IgG (30000-0-AP), and 30 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HEK-293-Zellen, HEK-293T-Zellen
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen
• Erfolgreiche ChIP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:300-1:1200
• CHImmunpräzipitation (IP): CHIP : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27740-1-AP bindet in WB, IF/ICC, ChIP, ELISA Histone H2B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Histone H2B fusion protein Ag26901
• Vollständiger Name: histone cluster 3, H2bb
• Beobachtetes Molekulargewicht: 15-20 kDa
• GenBank-Zugangsnummer: BC100855
• Gene symbol: Histone H2B
• Gene ID (NCBI): 128312
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST3H2BB is a replication-dependent histone that is a member of the histone H2B family.