HJURP Polyklonaler Antikörper

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• KD/KO Validated

HJURP Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Rezensionen (1)

Kat-Nr. : 15283-1-AP

Synonyme

FAKTS, FLEG1, hFLEG1, HJURP, Up regulated in lung cancer 9, URLC9

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  WB analysis using 15283-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15283-1-AP (HJURP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 15283-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15283-1-AP (HJURP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 15283-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15283-1-AP (HJURP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, A549-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 3 publications below

Produktinformation

15283-1-AP bindet in WB, IHC, ELISA HJURP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HJURP fusion protein Ag7402
• Vollständiger Name: Holliday junction recognition protein
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC001940
• Gene symbol: HJURP
• Gene ID (NCBI): 55355
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Holliday Junction Recognition Protein (HJURP, also known as hFLEG1) is a centromeric protein with a pivotal role in the incorporation and maintenance of histone H3-like variant Centromere Protein-A (CENP-A) in centromeres. It is a specific chaperone for CENP-A and it integrates newly synthesized CENP-A molecules and nucleosomes at replicated centromeres. (PMID: 28819432, PMID: 31492860)

Publikationen

Species	Application	Title
human	WB	Dev Cell; CENP-A Ubiquitylation Is Indispensable to Cell Viability.; Authors - Yohei Niikura
human	IHC	Front Cell Dev Biol; Comprehensive Analysis Uncovers Prognostic and Immunogenic Characteristics of Cellular Senescence for Lung Adenocarcinoma.; Authors - Weihao Lin
human	IHC	Int J Cancer; Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.; Authors - Fangqiu Fu
human	WB	J Biol Chem; Mitotic regulator Mis18β interacts with and specifies the centromeric assembly of molecular chaperone HJURP.; Authors - Jianyu Wang ; KD Validated
human	WB	Biochem Biophys Res Commun; Hypomethylation-driven overexpression of HJURP promotes progression of hepatocellular carcinoma and is associated with poor prognosis.; Authors - Ye Li ; KD Validated
		J Vis Exp; Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A).; Authors - Yohei Niikura

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christoffer (Verified Customer) (03-03-2025)

Adult mouse retina paraffin embedded and Cryo embedded embryonic (E14) mouse. The Cryo embedded tissue stains better with antigen retrieval (citric buffer).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Embryonic (E14) mouse retina and adult mouse retina.