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HJURP Monoklonaler Antikörper

HJURP Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F8C5

Kat-Nr. : 68995-1-PBS

Synonyme

1F8C5, FAKTS, Fetal liver-expressing gene 1 protein, FLEG1, hFLEG1

Filter:
  • Western Blot (WB) analysis of various lysates using HJURP Monoclonal antibody (68995-1-Ig)

    WB analysis using 68995-1-Ig (same clone as 68995-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68995-1-Ig (HJURP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68995-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using HJURP Monoclonal antibody (68995-1-Ig)

    IF Staining of A549 using 68995-1-Ig (same clone as 68995-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using HJURP antibody (68995-1-Ig, Clone: 1F8C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68995-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68995-1-PBS bindet in WB, IF/ICC, Indirect ELISA HJURP und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen HJURP fusion protein Ag34571
Vollständiger Name Holliday junction recognition protein
Berechnetes Molekulargewicht 86 kDa
Beobachtetes Molekulargewicht85-90 kDa
GenBank-ZugangsnummerBC001940
Gene symbol HJURP
Gene ID (NCBI) 55355
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Holliday Junction Recognition Protein (HJURP, also known as hFLEG1) is a centromeric protein with a pivotal role in the incorporation and maintenance of histone H3-like variant Centromere Protein-A (CENP-A) in centromeres. It is a specific chaperone for CENP-A and it integrates newly synthesized CENP-A molecules and nucleosomes at replicated centromeres. (PMID: 28819432, PMID: 31492860)