Hexokinase 1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Hexokinase 1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(58)

Rezensionen (2)

Kat-Nr. : 19662-1-AP

Synonyme

HK1, Hexokinase-A, Hexokinase-1, Hexokinase1, Hexokinase type I

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  WB analysis using 19662-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19662-1-AP (Hexokinase 1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19662-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19662-1-AP (Hexokinase 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19662-1-AP

  IP result of anti-Hexokinase 1 (IP:19662-1-AP, 4ug; Detection:19662-1-AP 1:2000) with HeLa cells lysate 1720 ug.

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  IHC staining of mouse brain using 19662-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19662-1-AP (Hexokinase 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 19662-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19662-1-AP (Hexokinase 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 19662-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 19662-1-AP (Hexokinase 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 19662-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Hexokinase 1 antibody (19662-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, Maushirngewebe, Rattenhirngewebe, THP-1-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Magenkrebsgewebe, humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 55 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

19662-1-AP bindet in WB, IF/ICC, IP, ELISA Hexokinase 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Hexokinase 1 fusion protein Ag8015
• Vollständiger Name: hexokinase 1
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC008730
• Gene symbol: Hexokinase 1
• Gene ID (NCBI): 3098
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The hexokinases (HKs) catalyze the first obligatory step in glucose metabolism to generate glucose-6-phosphate. This not only furthers glucose entry by maintaining the concentration gradient for facilitated glucose influx but also provides the first intermediate for essentially all major pathways using glucose. There are four conventional HK isoforms, HK1/2/3/4, encoded by four different genes. Most adult tissues express only HK1. Muscle and adipose tissue use HK2 for glycolysis, liver, and pancreatic beta cells express HK4 (also called glucokinase) and do not express HK1 or HK2. (PMID: 31434645, PMID: 31848318)

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Dual impacts of serine/glycine-free diet in enhancing antitumor immunity and promoting evasion via PD-L1 lactylation; Authors - Huan Tong
human	WB	Cell Stem Cell; Generation of human alveolar epithelial type I cells from pluripotent stem cells; Authors - Claire L Burgess
mouse	WB	Nat Immunol; NF-κB-inducing kinase maintains T cell metabolic fitness in antitumor immunity.; Authors - Meidi Gu
human,mouse	WB,IP,IF	Sci Adv; Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease.; Authors - Xinwen Zhang
human	WB	Nat Commun; SUMOylation controls the binding of hexokinase 2 to mitochondria and protects against prostate cancer tumorigenesis.; Authors - Xun Shangguan
pig	WB	Br J Pharmacol; PMID: 30515768; Authors - Xiaoying Lian

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wei (Verified Customer) (01-30-2020)

Strong, clear band for mouse heart.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Mouse Heart

FH

SCOTT (Verified Customer) (11-20-2018)

Used 1 in/1000 in 5% BSA TBS-Tween overnight at 4 C rolling. The membrane was blocked in 5% BSA TBS-Tween prior to adding the antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human induced neuronal progenitor derived astrocytes

Hexokinase 1 Antibody Western Blot, validation (1/1000 dilution) in Human induced neuronal progenitor derived astrocytes (Cat no:19662-1-AP)