Hexokinase 2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Hexokinase 2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (5)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(328)

Rezensionen (4)

Kat-Nr. : 22029-1-AP

Synonyme

HK2, EC:2.7.1.1, Hexokinase type II, hexokinase2, Hexokinase-2

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• 

  WB analysis using 22029-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22029-1-AP (Hexokinase 2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 22029-1-AP

  IP result of anti-Hexokinase 2 (IP:22029-1-AP, 4ug; Detection:22029-1-AP 1:10000) with HeLa cells lysate 1520 ug.

• 

  IHC staining of human urothelial carcinoma using 22029-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 22029-1-AP (Hexokinase 2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 22029-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Hexokinase 2 antibody (22029-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen, Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe, RAW 264.7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Urothelkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:20000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 12 publications below
• WB: See 299 publications below
• IHC: See 46 publications below
• IF: See 19 publications below
• IP: See 3 publications below
• CoIP: See 5 publications below
• RIP: See 1 publications below

Produktinformation

22029-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA Hexokinase 2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Huhn, Hund, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Hexokinase 2 fusion protein Ag16895
• Vollständiger Name: hexokinase 2
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 102-120 kDa
• GenBank-Zugangsnummer: BC021116
• Gene symbol: Hexokinase 2
• Gene ID (NCBI): 3099
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236). The antibody also recognizes Hexokinase 1.

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
mouse,human	WB	Cell Metab; An Upstream Open Reading Frame in Phosphatase and Tensin Homolog Encodes a Circuit Breaker of Lactate Metabolism.; Authors - Nunu Huang
mouse	WB	Cell Metab; STAT3 Activation-Induced Fatty Acid Oxidation in CD8+ T Effector Cells Is Critical for Obesity-Promoted Breast Tumor Growth.; Authors - Chunyan Zhang
	IP	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
mouse	WB	Nat Neurosci; Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex.; Authors - Xiaoxiang Dong
human	WB	Nat Commun; Parvimonas micra promotes oral squamous cell carcinoma metastasis through TmpC-CKAP4 axis; Authors - Houbao Qi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

SONAM (Verified Customer) (02-22-2024)

Works well

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cancer cells, macrophages

FH

Maialen (Verified Customer) (10-03-2023)

It works very well

• Applications: Immunoprecipitation

Hexokinase 2 Antibody Immunoprecipitation validation ( dilution) in (Cat no:22029-1-AP)

FH

Macarena (Verified Customer) (10-07-2022)

excellent results. Very specific

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cell lines

FH

Henar (Verified Customer) (08-31-2022)

Great antibody! No background and very specific result.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Primary mouse neurons