Hexokinase 2 Rekombinanter Antikörper

Hexokinase 2 Rekombinant Antikörper für WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250190C10

Kat-Nr. : 85875-1-PBS

Synonyme

HK2, EC:2.7.1.1, Hexokinase type II, hexokinase2, Hexokinase-2

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  WB analysis using 85875-1-RR (same clone as 85875-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85875-1-RR (Hexokinase 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85875-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85875-1-RR (same clone as 85875-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85875-1-RR (Hexokinase 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85875-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02173-3

  Sandwich ELISA standard curve of MP02173-3, Human Hexokinase 2 Recombinant Matched Antibody Pair - PBS only. 85875-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16895. 85875-1-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

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  Sample test of MP02173-3

  The mean Hexokinase 2 concentration was determined to be 321.70 ng/mL in A549 cell extract based on a 1.3 mg/mL extract load.

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  Sample test of MP02173-3

  Serum of sixteen individual healthy human donors was measured. The human Hexokinase 2 concentration of detected samples was determined to be 1.16 ng/mL with a range of 0.35 - 4.27 ng/mL.

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  IHC staining of mouse skeletal muscle using 85875-1-RR (same clone as 85875-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 85875-1-RR (Hexokinase 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85875-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 85875-1-RR (same clone as 85875-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 85875-1-RR (Hexokinase 2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85875-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85875-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85875-1-RR against Human Hexokinase 2 were performed. The affinity constant is 0.302 nM.

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Geprüfte Anwendungen

Produktinformation

85875-1-PBS bindet in WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test Hexokinase 2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Hexokinase 2 fusion protein Ag16895
• Vollständiger Name: hexokinase 2
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 102-120 kDa
• GenBank-Zugangsnummer: BC021116
• Gene symbol: Hexokinase 2
• Gene ID (NCBI): 3099
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236).