HK3 Monoklonaler Antikörper

HK3 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3H3C7

Kat-Nr. : 67803-1-PBS

Synonyme

EC:2.7.1.1, Hexokinase 3, hexokinase 3 (white cell), Hexokinase type III, Hexokinase-3

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  WB analysis of human saliva using 67803-1-Ig (same clone as 67803-1-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

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  WB analysis of rat spleen using 67803-1-Ig (same clone as 67803-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 67803-1-Ig (same clone as 67803-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 67803-1-Ig (same clone as 67803-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 67803-1-Ig (same clone as 67803-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67803-1-Ig (same clone as 67803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67803-1-Ig (HK3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67803-1-Ig (same clone as 67803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67803-1-Ig (HK3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67803-1-PBS bindet in WB, IHC, ELISA HK3 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HK3 fusion protein Ag30798
• Vollständiger Name: hexokinase 3 (white cell)
• Berechnetes Molekulargewicht: 923 aa, 99 kDa
• Beobachtetes Molekulargewicht: 99 kDa
• GenBank-Zugangsnummer: BC028129
• Gene symbol: HK3
• Gene ID (NCBI): 3101
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hexokinases phosphorylate glucose to produce glucose-6-phosphate (G6P), the first step in most glucose metabolism pathways. Hexokinase 3 also known as HK3, similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate.