HLA class I ABC Polyklonaler Antikörper
HLA class I ABC Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human
Anwendung
WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA, Cell treatment
Konjugation
Unkonjugiert
Kat-Nr. : 15240-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, L02-Zellen, NCI-H1299-Zellen |
| Erfolgreiche IP | HepG2-Zellen |
| Erfolgreiche Detektion in IHC | humanes Tonsillitisgewebe, humanes Magenkrebsgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | HepG2-Zellen, Raji-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:5000-1:20000 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 36 publications below |
| IHC | See 20 publications below |
| IF | See 16 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
| RIP | See 1 publications below |
Produktinformation
15240-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA, Cell treatment HLA class I ABC und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | HLA class I ABC fusion protein Ag7370 |
| Vollständiger Name | major histocompatibility complex, class I, A |
| Berechnetes Molekulargewicht | 41 kDa |
| Beobachtetes Molekulargewicht | 44 kDa |
| GenBank-Zugangsnummer | BC003069 |
| Gene symbol | HLA-A |
| Gene ID (NCBI) | 3105 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for HLA class I ABC antibody 15240-1-AP | Protokoll herunterladen |
| IHC protocol for HLA class I ABC antibody 15240-1-AP | Protokoll herunterladenl |
| IF protocol for HLA class I ABC antibody 15240-1-AP | Protokoll herunterladen |
| IP protocol for HLA class I ABC antibody 15240-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Cell IRGQ-mediated autophagy in MHC class I quality control promotes tumor immune evasion | ||
Signal Transduct Target Ther Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis | ||
Cell Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy. | ||
Cancer Discov Spatial-Temporal Diversity of Extrachromosomal DNA Shapes Urothelial Carcinoma Evolution and the Tumor Immune Microenvironment | ||
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH SHIVA KRISHNA (Verified Customer) (08-06-2025) | I used this antibody for western blotting, and it worked perfectly.
|
FH Fabien (Verified Customer) (11-04-2022) | The antibody works great for western-blot.
|
FH Patricia (Verified Customer) (02-25-2020) | I tried probing my WB for 1 hour at RT, but it looked terrible, so I will try an overnight incubation. I've also tried my IP twice, once using 4 ug/mL, and the second time using 10 ug/mL. I had no pulldown either time. I'm quite disappointed.
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