HLA class I ABC Polyklonaler Antikörper

HLA class I ABC Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA, Cell treatment

Konjugation

Unkonjugiert

Publikationen(64)

Kat-Nr. : 15240-1-AP

Synonyme

HLA-A, HLA A, HLA class I, HLA class I (HLA-A), HLA class I histocompatibility antigen, A alpha chain



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, L02-Zellen, NCI-H1299-Zellen
Erfolgreiche IPHepG2-Zellen
Erfolgreiche Detektion in IHChumanes Tonsillitisgewebe, humanes Magenkrebsgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen, Raji-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:5000-1:20000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

15240-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA, Cell treatment HLA class I ABC und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen HLA class I ABC fusion protein Ag7370
Vollständiger Name major histocompatibility complex, class I, A
Berechnetes Molekulargewicht 41 kDa
Beobachtetes Molekulargewicht 44 kDa
GenBank-ZugangsnummerBC003069
Gene symbol HLA-A
Gene ID (NCBI) 3105
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for HLA class I ABC antibody 15240-1-APProtokoll herunterladen
IHC protocol for HLA class I ABC antibody 15240-1-APProtokoll herunterladenl
IF protocol for HLA class I ABC antibody 15240-1-APProtokoll herunterladen
IP protocol for HLA class I ABC antibody 15240-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell

IRGQ-mediated autophagy in MHC class I quality control promotes tumor immune evasion

Authors - Lina Herhaus
humanWB

Nature

Inhibition of PCSK9 potentiates immune checkpoint therapy for cancer.

Authors - Xinjian Liu
humanWB

Signal Transduct Target Ther

Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis

Authors - Chuan Yang
humanWB

Cell

Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy.

Authors - Kazuhiro Koikawa
humanIHC

Cancer Discov

Spatial-Temporal Diversity of Extrachromosomal DNA Shapes Urothelial Carcinoma Evolution and the Tumor Immune Microenvironment

Authors - Wei Lv
human

Nat Cell Biol

An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.

Authors - James W Clancy

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

SHIVA KRISHNA (Verified Customer) (08-06-2025)

I used this antibody for western blotting, and it worked perfectly.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Small cell lung cancer
FH

Fabien (Verified Customer) (11-04-2022)

The antibody works great for western-blot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: Jy cells
FH

Patricia (Verified Customer) (02-25-2020)

I tried probing my WB for 1 hour at RT, but it looked terrible, so I will try an overnight incubation. I've also tried my IP twice, once using 4 ug/mL, and the second time using 10 ug/mL. I had no pulldown either time. I'm quite disappointed.

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000 for WB; 4-10 ug/mL IP
  • Cell Tissue Type: Ovarian cancer