HLA-A Polyklonaler Antikörper

HLA-A Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 55383-1-AP

Synonyme

HLA A, HLA class I, HLA class I (HLA-A), HLA class I ABC, HLA class I histocompatibility antigen, A alpha chain

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  WB analysis using 55383-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55383-1-AP (HLA-A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 55383-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55383-1-AP (HLA-A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 55383-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55383-1-AP (HLA-A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 55383-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 55383-1-AP (HLA-A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 55383-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 55383-1-AP (HLA-A Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 55383-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using HLA-A antibody (55383-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

55383-1-AP bindet in WB, IHC, IF/ICC, ELISA HLA-A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: major histocompatibility complex, class I, A
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 41-44 kDa
• GenBank-Zugangsnummer: NM_002116
• Gene symbol: HLA-A
• Gene ID (NCBI): 3105
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Publikationen

Species	Application	Title
human	IF	J Virol; Two Functional Variants of AP-1 Complexes Composed of either γ2 or γ1 Subunits Are Independently Required for Major Histocompatibility Complex Class I Downregulation by HIV-1 Nef.; Authors - Lucas A Tavares
human	IHC	Front Pharmacol; Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosis; Authors - Yanling Wang