HLA-DMB Rekombinanter Antikörper

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• KD/KO Validated

HLA-DMB Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

230123A3

Kat-Nr. : 82922-1-RR

Synonyme

RING7, MHC class II antigen DMB, HLA DMB, DMB, 230123A3

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  WB analysis of Daudi using 82922-1-RR

  WB result of HLA-DMB antibody (82922-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HLA-DMB transfected Daudi cells.

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  WB analysis using 82922-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82922-1-RR (HLA-DMB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 82922-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82922-1-RR (HLA-DMB antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 82922-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82922-1-RR (HLA-DMB antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Raji using 82922-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DMB antibody (82922-1-RR, Clone: 230123A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  Affinity and Kinetic Characterization of 82922-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82922-1-RR against Human HLA-DMB were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, Daudi-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82922-1-RR bindet in WB, IHC, IF/ICC, ELISA HLA-DMB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HLA-DMB fusion protein Ag16320
• Vollständiger Name: major histocompatibility complex, class II, DM beta
• Berechnetes Molekulargewicht: 263 aa, 29 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC027175
• Gene symbol: HLA-DMB
• Gene ID (NCBI): 3109
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides.