HLA-DR Rekombinanter Antikörper

HLA-DR Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

L243

Kat-Nr. : 65560-1-MR

Synonyme

HLA-DRA, HLA DRA, HLA DRA1, HLA DRB, HLA-DRA1

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  FC experiment of human PBMCs using 65560-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human HLA-DR Mouse IgG2a Recombinant Antibody (65560-1-MR, Clone: L243) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 65560-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human HLA-DR Mouse IgG2a Recombinant Antibody (65560-1-MR, Clone: L243)(red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65560-1-MR bindet in FC HLA-DR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Human lymphoblastoid B-cell line RPMI 8866
• Vollständiger Name: major histocompatibility complex, class II, DR alpha
• Berechnetes Molekulargewicht: 254 aa, 29 kDa
• GenBank-Zugangsnummer: BC032350
• Gene symbol: HLA-DRA
• Gene ID (NCBI): 3122
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

HLA-DR is a member of MHC class II molecules encoded by the human leukocyte antigen complex on the region 6p21.31 of chromosome 6. HLA-DR is composed of two non-covalently associated transmembrane glycoproteins (the α and β chains), and is primarily expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelial cells (PMID: 33125816). HLA-DR participates in the antigen presentation to CD4+ or CD8+ T cells and is postulated as a general biomarker of sepsis-induced immunosuppression (PMID: 28199206, 32421903).