HLA-DR Rekombinanter Antikörper

HLA-DR Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

L243

Kat-Nr. : 65560-1-PBS

Synonyme

HLA-DRA, HLA DRA, HLA DRA1, HLA DRB, HLA-DRA1

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  FC experiment of human PBMCs using 65560-1-MR (same clone as 65560-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human HLA-DR Mouse IgG2a Recombinant Antibody (65560-1-MR, Clone: L243) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65560-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65560-1-MR (same clone as 65560-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human HLA-DR Mouse IgG2a Recombinant Antibody (65560-1-MR, Clone: L243)(red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65560-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

65560-1-PBS bindet in FC HLA-DR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Human lymphoblastoid B-cell line RPMI 8866
• Vollständiger Name: major histocompatibility complex, class II, DR alpha
• Berechnetes Molekulargewicht: 254 aa, 29 kDa
• GenBank-Zugangsnummer: BC032350
• Gene symbol: HLA-DRA
• Gene ID (NCBI): 3122
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. Stable for one year after shipment.

Hintergrundinformationen

HLA-DR is a member of MHC class II molecules encoded by the human leukocyte antigen complex on the region 6p21.31 of chromosome 6. HLA-DR is composed of two non-covalently associated transmembrane glycoproteins (the α and β chains), and is primarily expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelial cells (PMID: 33125816). HLA-DR participates in the antigen presentation to CD4+ or CD8+ T cells and is postulated as a general biomarker of sepsis-induced immunosuppression (PMID: 28199206, 32421903).