HLA-DR Rekombinanter Antikörper

HLA-DR Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

L243

Kat-Nr. : CL750-65560

Synonyme

HLA-DRA, HLA DRA, HLA DRA1, HLA DRB, HLA-DRA1

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  FC experiment of human PBMCs using CL750-65560

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD14 and 5 ul CoraLite® Plus 750 Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (CL750-65560, Clone: L243), or CoraLite® Plus 750 Mouse IgG2a Isotype Control (C1.18.4) (CL750-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL750-65560

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (CL750-65560, Clone: L243) (red), or CoraLite® Plus 750 Mouse IgG2a Isotype Control (C1.18.4) (CL750-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL750-65560 bindet in FC HLA-DR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Human lymphoblastoid B-cell line RPMI 8866
• Vollständiger Name: major histocompatibility complex, class II, DR alpha
• Berechnetes Molekulargewicht: 254 aa, 29 kDa
• GenBank-Zugangsnummer: BC032350
• Gene symbol: HLA-DRA
• Gene ID (NCBI): 3122
• Konjugation: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

HLA-DR is a member of MHC class II molecules encoded by the human leukocyte antigen complex on the region 6p21.31 of chromosome 6. HLA-DR is composed of two non-covalently associated transmembrane glycoproteins (the α and β chains), and is primarily expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelial cells (PMID: 33125816). HLA-DR participates in the antigen presentation to CD4+ or CD8+ T cells and is postulated as a general biomarker of sepsis-induced immunosuppression (PMID: 28199206, 32421903).