HLA-DRA Polyklonaler Antikörper

HLA-DRA Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 17221-1-AP

Synonyme

MHC class II antigen DR, HLA-DRA1, HLA-DR, HLA DRB, HLA DRA1

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  WB analysis using 17221-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17221-1-AP (HLA-DRA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 17221-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 17221-1-AP (HLA-DRA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 17221-1-AP

  IP result of anti-HLA-DRA (IP:17221-1-AP, 4ug; Detection:17221-1-AP 1:1000) with Raji cells lysate 3200 ug.

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  IHC staining of human tonsillitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17221-1-AP (HLA-DRA Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human tonsillitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17221-1-AP (HLA-DRA Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human appendicitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17221-1-AP (HLA-DRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17221-1-AP (HLA-DRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17221-1-AP (HLA-DRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17221-1-AP (HLA-DRA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Raji using 17221-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRA antibody (17221-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, Raji-Zellen, Ramos-Zellen, Rattenmilzgewebe
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Appendizitis-Gewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Produktinformation

17221-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA HLA-DRA und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HLA-DRA fusion protein Ag11044
• Vollständiger Name: major histocompatibility complex, class II, DR alpha
• Berechnetes Molekulargewicht: 254 aa, 29 kDa
• Beobachtetes Molekulargewicht: 32-34 kDa
• GenBank-Zugangsnummer: BC032350
• Gene symbol: HLA-DRA
• Gene ID (NCBI): 3122
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Nat Commun; Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma.; Authors - Zhaohui Chen
rat	IF	Aging Cell; Donor BMSC-derived small extracellular vesicles relieve acute rejection post-renal allograft through transmitting Loc108349490 to dendritic cells.; Authors - Zhi-Gang Wang
human	WB	Front Immunol; An Immunological Perspective: What Happened to Pregnant Women After Recovering From COVID-19?; Authors - Sijia Zhao
human	IF	Front Immunol; Functional Change of Effector Tumor-Infiltrating CCR5+CD38+HLA-DR+CD8+ T Cells in Glioma Microenvironment.; Authors - Pin-Yuan Chen
rat	IHC	Mol Med; Correction of immunosuppression in aged septic rats by human ghrelin and growth hormone through the vagus nerve-dependent inhibition of TGF-β production.; Authors - Mian Zhou
human	IHC	BMC Cancer; HLA class II molecule HLA-DRA identifies immuno-hot tumors and predicts the therapeutic response to anti-PD-1 immunotherapy in NSCLC.; Authors - Jie Mei