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HLA-DRB4 Rekombinanter Antikörper

HLA-DRB4 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230352F10

Kat-Nr. : 83103-1-PBS

Synonyme

MHC class II antigen DRB4, HLA DRB4, HLA DR4B, 230352F10

Filter:
  • Western Blot (WB) analysis of various lysates using HLA-DRB4 Recombinant antibody (83103-1-RR)

    WB analysis using 83103-1-RR (same clone as 83103-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83103-1-RR (HLA-DRB4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83103-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-DRB4 Recombinant antibody (83103-1-RR)

    IHC staining of human tonsillitis using 83103-1-RR (same clone as 83103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83103-1-RR (HLA-DRB4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83103-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using HLA-DRB4 Recombinant antibody (83103-1-RR)

    IF Staining of Raji using 83103-1-RR (same clone as 83103-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRB4 antibody (83103-1-RR, Clone: 230352F10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83103-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83103-1-RR

    Affinity and Kinetic Characterization of 83103-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83103-1-RR against Human HLA-DRB4 were performed. The affinity constant is 0.623 nM.

Geprüfte Anwendungen

Produktinformation

83103-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HLA-DRB4 und zeigt Reaktivität mit Hausschwein, human

Getestete Reaktivität Hausschwein, human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen HLA-DRB4 fusion protein Ag8492
Vollständiger Name major histocompatibility complex, class II, DR beta 4
Berechnetes Molekulargewicht 266 aa, 30 kDa
Beobachtetes Molekulargewicht30 kDa
GenBank-ZugangsnummerBC005312
Gene symbol HLA-DRB4
Gene ID (NCBI) 3126
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.